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Fluorescence intensity of each channel was assessed by automated generation of cell outline around identified nuclei, using the ZenDesk 2D image analysis toolkit, as represented ( a ). Expanded data from . Apoptotic (c-Casp-3 + ) cell populations defined by expression of CD45 and PLIN2, expressed as percentage of total plaque cells. Data as mean ± SEM, # P ≤ 0.1, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Journal: bioRxiv

Article Title: Postoperative Stress Accelerates Atherosclerosis through Inflammatory Remodeling of the HDL Proteome and Impaired Reverse Cholesterol Transport

doi: 10.1101/2025.05.02.651357

Figure Lengend Snippet: Fluorescence intensity of each channel was assessed by automated generation of cell outline around identified nuclei, using the ZenDesk 2D image analysis toolkit, as represented ( a ). Expanded data from . Apoptotic (c-Casp-3 + ) cell populations defined by expression of CD45 and PLIN2, expressed as percentage of total plaque cells. Data as mean ± SEM, # P ≤ 0.1, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Article Snippet: Using the ZEISS Zendesk 2D image analysis toolkit, we quantified the per-cell expression of CD45, PLIN2 and cleaved (active) caspase-3 (c-Casp-3), an apoptosis marker ( , ).

Techniques: Fluorescence, Expressing